|
|
|
Geen items |
Cells and Tissue DNA Isolation Micro Kit
Product code : NOR.57300
NOR.57300Optimized for small inputs of cells and tissues, such as Laser-Captured Microdissection (LCM)
Via de knop “offerte aanvraag” kan u vrijblijvend contact met ons opnemen voor verdere informatie.
Cells and Tissue DNA Isolation Micro Kit
Product beschrijving
- Optimized for small inputs of cells and tissues, such as Laser-Captured Microdissection (LCM)
- DNA is eluted in small elution volumes (20 - 40µL)
- Fast and easy processing using a rapid spin-column format
- Isolate high quality genomic DNA
Norgen's Cells and Tissue DNA Isolation Micro Kit is designed for the rapid preparation of genomic DNA from low inputs of cultured cells as well as various tissue samples. This kit is optimized to isolate DNA from small inputs of cells and tissues, such as Laser-Captured Microdissection (LCM) samples. The genomic DNA is preferentially purified from other cellular proteinaceous components, and is eluted into a small elution volume. Typical yields of genomic DNA will vary depending on the sample being processed. Preparation time for a single sample is approximately 60 minutes, and each kit contains sufficient materials for 50 preparations.
|
Kit Specifications
|
|
|
Maximum Tissue Input
|
3 mg animal tissue
Up to 150µL of viral suspension 5 x 10 5 cells |
|
Column Loading Capacity
|
> 750µL
|
|
Elution Volume
|
20 - 40µL |
| Analyte Purified | Genomic DNA, mitochondrial DNA, viral DNA |
| Time to Complete Purification |
60 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers. The kit contains a ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for up to 1 year after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2-8°C is recommended.
| Component | Cat. 57300 (50 preps) |
|---|---|
| Lysis Buffer B | 20 mL |
| Solution WN | 18 mL |
| Wash Solution A | 18 mL |
| Elution Buffer B | 8 mL |
| Proteinase K | 1.2 mL |
| Micro Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
Cells and Tissue DNA Isolation Micro Kit
Product specificaties
Packaging Unit
50 preps
Brand
Norgen biotek
Category
Genomic DNA Purification Kits
Purification
DNA Purification