VIROTPING KIT PCV2 a,b,d

VIROTPING KIT PCV2 a,b,d

Product beschrijving

Product description:

ViroTyping Kit PCV2 a, b, d contains three PCR primer sets for the amplification and detection of DNA of the porcine circovirus type 2 (PCV2) a, b, d. This test is based on an intercalating dye using real-time PCR and was developed and validated for the Applied Biosystems® 7500 (Fast) instrument (Thermo Fisher Scientific), but is also suitable for other real-time PCR instruments. This test allows the rapid typing of PCV2 a, b, d from DNA samples.
ViroTyping Kit PCV2 a, b, d detects the capsid gene of porcine circovirus type 2 (PCV2) a, b, d. The test is based on phylogenetic analysis and the definition of genotype-specific marker positions for PCV2 genotypes, published by Franzo and colleagues. Amplification curves and specific melting curves for PCV2 a, b and d indicate the amplification of genotype specific DNA.
When using PCR-platforms not validated by ingenetix, an evaluation of the real-time PCR is recommended. Please be aware that some PCR-platforms have to be calibrated with the corresponding dye before performing PCR.

Pathogen information:

Porcine circovirus type 2 (PCV2) is a member of the family Circoviridae, genus Circovirus and is an icosahedral, non-enveloped DNA virus with a single-stranded ambisense circular genome.
PCV2 infection with postweaning multisystemic wasting syndrome (PMWS) occurs all over the world. There is evidence that genetic diversity is affecting the virulence of PCV2. At the intraspecific level, two major PCV2 groups (PCV2a and PCV2b) were initially defined, followed by a third genotype named PCV2c and a fourth group, named PCV2d.
The ORF2 sequences enable differentiation of the genotypes PCV2a, PCV2b and PCV2d.

Principle of Real Time PCR:

A specific DNA sequence of the pathogen genome is amplified by a set of two PCR primers that flank the target region and the generated PCR-product is detected by an intercalating nucleic acid dye with subsequent melt curve analysis.