BactoReal® Kit Borrelia burgdorferi sensu lato

BactoReal® Kit Borrelia burgdorferi sensu lato

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Kit contents:

• Detection assay for Borrelia burgdorferi sensu lato
• Detection assay for internal positive control (control of amplification)
• DNA reaction mix (contains uracil-N glycosylase, UNG)
• Positive control for Borrelia
• Water

Background: Borrelia are Gram-negative bacteria belonging to the Spirochaetaceae. Borrelia burgdorferi sensu stricto, B. garinii, B. afzelii, B. valaisiana, B. lusitaniae and B. spielmanii belong to the B. burgdorferi sensu lato-complex and cause the Lyme disease (Lyme borreliosis). These species are associated with different genospecies specific symptoms. They are transmissed by ticks, mainly by Ixodes ricinus. The Lyme disease is a multisystemic illness, which can affect various organs, especially the nervous system, skin, heart and joints.

Description: BactoReal^® Kit Borrelia burgdorferi sensu lato is based on the amplification and detection of the ospA gene of Borrelia species belonging to the Borrelia burgdorferi sensu lato complex using real-time PCR.

PCR-platforms: BactoReal^® Kit Borrelia burgdorferi sensu lato is developed and validated for the ABI PRISM^® 7500 instrument (Life Technologies), LightCycler^® 480 (Roche) and Mx3005P^® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description