ViroReal® Kit SARS-CoV-2 & SARS

ViroReal® Kit SARS-CoV-2 & SARS

Product beschrijving

Background: 

SARS-CoV-2 is a beta coronavirus that emerged in Wuhan, China in December 2019 and can lead to pneumonia. Fever, cough and breathing difficulties are described as the most frequent initial symptoms. According to the WHO, the possibility of human-to-human transmission has been proven. An exact clarification of the mode of transmission is still in progress. In addition, the WHO states that so far, a mild course of infection has been observed in most of the cases, while more severe courses were observed in about 20% of the cases.

Description: 

ViroReal® Kit SARS-CoV-2 & SARS uses RT real-time PCR for the detection of RNA of the n-gene of coronaviruses SARS-CoV-2 and SARS . Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogens as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well. For more detailed information see here

Product Features:

• Amplification and detection: n-gene of SARS-CoV-2 and SARS
• Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
• contains RNase Inhibitor to block RNA degradation
• Real-time PCR with rapid hot-start Taq DNA polymerase
• ROX™ dye as passive reference
• Internal Positive Control System to exclude false-negative results
• Optimized to handle PCR inhibitors
• PCR- platforms: runs on all established standard real-time PCR- platforms
• Harmonized thermal profiles to run RNA and DNA samples simultaneously
• Available formats: Kit (with reaction mix)

PCR platforms: ViroReal® Kit SARS-CoV-2 & SARS is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.