1Step RT qPCR Green ROX H Kit

1Step RT qPCR Green ROX H Kit

Product description

highQu 1Step RT qPCR mastermixes in combination with a blend of thermostable and extremely active Reverse Transcriptase & advanced RNase Inhibitor (RT Mix) allow for a single step one tube RT qPCR.

qPCR mastermixes are based on the small molecular inhibitor technology Hot Start PCR allowing to achieve highest sensitivity and specificity under both standard and fast qPCR cycling conditions. They provide excellent results on both AT/GC rich templates and guaranty rapid extension with early Ct values with minimum or no optimization.

Our mastermixes are supplied with PCR Water to guaranty the best performance. To suit the broad instrument range the 1Step RT qPCR Green Mixes are available in two versions – with low or high ROX concentration.

For optional use, the ROX passive reference dye is premixed within the ROX L and ROX H qPCR Mixes. If the purchaser has an instrument capable of optional ROX detection and wishes to perform the optional normalization of the signal, then the user must select the option in the software.

Notice to Purchaser: With purchasing of this product, no rights are conveyed with respect to U.S. Patent: 5,928,907 and corresponding patents outside the US.

Applications

• Dye-based RT-qPCR
• Relative gene expression analysis
• Quantification of mRNA, total RNA, viral RNA, low copy number genes

Benefits

• Efficient Reverse transcription & sensitive qPCR in one tube
• Both standard and fast cycling, GC rich templates
• Rapid extension, early Ct
• Supplied with PCR Water