ParoReal® Kit Histomonas meleagridis

ParoReal® Kit Histomonas meleagridis

Product description

Kit contents:

• Detection assay for Histomonas meleagridis
• Detection assay for internal positive control (control of amplification)
• DNA reaction mix (contains uracil-N glycosylase, UNG)
• Positive control for H. meleagridis
• Water

Background: Histomonas meleagridis is a parasitic protozoan causing blackhead disease, infectious enterohepatitis, or histomoniasis. It can infect turkeys, chickens, and occasionally other galliform birds but it is most deadly in turkeys. Chickens are more resistant to the effects of the infection but may act as carriers. Histomonas meleagridis induces extensive and severe necrosis of the tissues of the mucosa and submucosa of cecum and parenchyma of the liver. It is transmitted by the nematode Heterakis gallinarum or directly from the droppings of infected birds. Histomonas meleagridis interacts with other gut organisms, such as bacteria and coccidia, and depends on these for full virulence.

Description: ParoReal Kit Histomonas meleagridis is based on the amplification and detection of the 18S rRNA gene of H. meleagridis using real-time PCR.

PCR-platforms: ParoReal Kit Histomonas meleagridis is developed and validated for the ABI PRISM^® 7500 instrument (Life Technologies), LightCycler^® 480 (Roche) and Mx3005P^® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description