VIROREAL KIT PCV3

VIROREAL KIT PCV3

Product beschrijving

Kit contents:

• Detection assay for CAEV (caprine arthritis and encephalitis virus)
• Detection assay + target for internal RNA positive control (control of RT-PCR amplification and/or RNA extraction)
• RNA reaction mix for one-step reverse transcription real-time PCR
• Nuclease-free water
• Positive control (DNA) for CAEV

Background:

The Caprine Arthritis and Encephalitis Virus (CAEV) of goats is an enveloped, single-stranded RNA small ruminant lentivirus (SRLV) in the family Retroviridae. There are several, genetically distinct isolates of the virus that differ in virulence. The CAE-virus is closely related to the Maedi-Visna-Virus (MVV) of sheep. Both infect cells of the monocyte/macrophage lineage and cause lifelong infections. Transmission of the CAEV is through ingestion of virus-infected goat milk but also through virus-infected secretion of affected animals and droplet infection. Clinical symptoms such as severe arthritis of the carpal joint are seen in approximately 20 % of infected goats. Subclinical or clinical interstitial pneumonia, mastitis with lower milk production and chronic wasting have also been attributed to infection with the virus.

Description:

ViroReal® Kit CAEV is based on the amplification and detection of the gag polyprotein gene by one-step reverse transcription real-time PCR (RT PCR). It allows the rapid and sensitive detection of CAEV RNA purified from blood (mononuclear cells, PBMC) (e.g. with the QIAamp Viral RNA Mini Kit, Qiagen).

 

PCR platforms:

ViroReal® Kit CAEV is developed for the Applied Biosystems® 7500 instrument (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.