Endoproteinase Glu-C (V8 proteinase), MS approved from Staphylococcus aureuslyophil.

Endoproteinase Glu-C (V8 proteinase), MS approved from Staphylococcus aureuslyophil.

Product Description

Endoproteinase Glu-C (V8) is a serine endoproteinase isolated from Staphylococcus aureus V8. Approved quality for use with in-gel digestion and mass spectrometric analysis. The specificity of Glu-C is primarily determined by the buffer pH and composition. Using phosphate buffers (pH 7.8), Glu-C will cleave at both glutamyl and aspartyl bonds. Ammonium bicarbonate buffer (pH 7.8) will lead to a preferential cleavage of glutamyl bonds. The presence of proline residues on the carboxy side of the peptide bond inhibits the cleavage. Due to its highly specific cleavage of peptides Glu-C is used in proteomics for peptide mapping and protein sequence work.